dna fingerprinting of bovine cryptosporidium isolates in qazvin province, iran

نویسندگان

احسان ناظم الحسینی مجرد

ehsan nazemalhosseini mojarad research center for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iranدانشگاه علوم پزشکی شهید بهشتی، مرکز تحقیقات بیماری های گوارش و کبد نیلوفر تقی پور

niloofare taghipour department of medical parasitology & mycology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iranدانشگاه علوم پزشکی شهید بهشتی، دانشکده پزشکی، گروه انگل شناسی و قارچ شناسی علی حقیقی

ali haghighi department of medical parasitology & mycology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran2- دانشگاه علوم پزشکی شهید بهشتی، دانشکده پزشکی، گروه انگل شناسی و قارچ شناسی اکبر کشاورز

akbar keshavarz 660 laboratory research center for office of health and treatment, tehran, iran3- اداره بهداشت و درمان نیروی زمینی ارتش جمهوری اسلامی ایران، مرکز تحقیقات آزمایشگاهی ۶۶۰ نزاجا محمد رستمی نژاد

چکیده

introduction: cryptosporidium is an apicomplexa parasite that infects humans and a wide range of domestic and wild animals. however, the sub-genotypes of the species infecting animals in iran are unclear. the aim of the present study isto identify dna fingerprinting of bovine cryptosporidium in qazvin province using sequences of gp60 gene.   materials and methods : in this study we investigated 25 c. parvum isolated form bovine in qazvin animal husbandries. subgenotypes were determined by dna sequencing of 60-kda glycoprotein gene.   results: using dna sequencing of gp60 gene, two subtype families within the c. parvum included iid (15/22) and iia (7/22) were recognized. also three subtypes in these two subtype families included iia a15g2r1 (22/25), iia a16g3r (1/25), iid a15g1 (2/25) were determined.   conclusion: today, new zoonose strains are identified which based on severity of infection compared with human strains are more sever and the source and transmission of their infection are unclear. therefore, determination of c .parvum strains, genotypes and sub-genotypes for epidemiological studies are quite necessary specially to identify the animal sources and to improve the control and prevention programs due to the lake of effective drugs for this infection.

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